Nickan Research Institute Immunopathologia Persa 2423-8015 8 1 2022 01 01 Secretome of adipose derived stem cells induced apoptosis in anaplastic thyroid carcinoma C-643 cells e20 e20 10.34172/ipp.2022.20 EN Fatemeh Rezaei-Tazangi https://orcid.org/0000-0003-1402-6482 Ali Samadi https://orcid.org/0000-0002-0489-6022 Saeed Azandeh https://orcid.org/0000-0002-5375-248X Saeed Khoshnood https://orcid.org/0000-0002-5143-3178 Shahab Mahmoudvand https://orcid.org/0000-0002-9155-9939 Journal Article 10.34172/ipp.2022.20 2021 07 28 2021 09 08 Introduction: Previous studies have proven that secretory materials of mesenchymal stem cells (MSCs) (secretome) have remedial properties. Objectives: The main goal of this study was to evaluate the effects of secretome of adipose-derived MSCs on growth and apoptosis in anaplastic thyroid carcinoma (ATC) C-643 cells. Materials and Methods: Initially, thyroid carcinoma cells were exposed to the 25 and 50 μg/mL adipose-derived stem cells (ADSCs) secretome for 24 and 48 hours, to evaluate the proliferation and cytotoxicity of the C-643 cells, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test and colony assay was conducted. Acridine orange/ethidium bromide (AO/EB) staining was conducted to evaluate the apoptosis. The expression level of apoptosis-associated genes was determined by real-time polymerase chain reaction (PCR) technique. Results: Cell viability and colony numbers in groups exposed to secretome were significantly lower than the control group. The amount of apoptosis-related genes (Bax/Bcl-2, P53, caspase-3 and caspase-8) expression in secretome-treated groups was more than the control group. Conclusion: The data revealed that ADSCs secretome caused the significant reduction of cell growth and induced apoptosis in C-643 cells.