Nickan Research Institute Immunopathologia Persa 2423-8015 11 2 2025 07 01 Exploring the therapeutic potential of Cerebrolysin-loaded exosomes in multiple sclerosis; an experimental study on neuroprotection and immune modulation e41735 e41735 10.34172/ipp.2025.41735 EN Mohammad Hassan Omrani https://orcid.org/0009-0006-2164-9951 Sina Khodakarimi https://orcid.org/0000-0002-0434-4180 Hanieh Beyrampour-Basmenj https://orcid.org/0000-0002-7919-0024 Mahnaz Talebi https://orcid.org/0000-0002-7613-3913 Naeimeh Akbari-Gharalari https://orcid.org/0009-0007-3724-2061 Abbas Karimi https://orcid.org/0000-0002-1172-8502 Zeynab Aliyari-Serej https://orcid.org/0000-0002-4355-1908 Maryam Eyvazi https://orcid.org/0009-0000-3810-7864 Ahmad Mehdipour https://orcid.org/0000-0002-1112-2439 Abbas Ebrahimi-Kalan https://orcid.org/0000-0001-5466-6754 Journal Article 10.34172/ipp.2025.41735 2024 08 12 2025 01 23 Introduction: Multiple sclerosis (MS) is a complex immune-mediated disorder of the central nervous system (CNS) characterized by inflammation and degeneration, currently lacking a cure. The blood-brain barrier (BBB) challenges of delivery of therapeutic agents to affected neural tissues. Exosomes, with low-immunogenicity and the ability to cross biological barriers, offer a promising avenue for targeted delivery of neurotrophic factors (NTFs) to support neuronal survival and regeneration. This study investigated the therapeutic potential of stem cell-derived exosomes (SCDEs) and Cerebrolysin-loaded exosomes (CLE) in an experimental autoimmune encephalomyelitis (EAE) model, a preclinical paradigm for MS. Objectives: The co-delivery of NTFs and exosomes aimed to modulate the immune response and promote remyelination and neuroprotection in EAE. Materials and Methods: An EAE modeling was induced in C57BL/6 mice in an experimental study, treated with SCDE, CBL, or CLE by intraperitoneal administration. Assessments included clinical scoring, gene expression analysis in splenocytes, and cytokine quantification. Results: Results showed that the CLE group exhibited significant improvement in behavioral manifestations compared to SCDE and CBL groups. CLE treatment stimulated survival signaling pathways, influencing EAE progression, and suppressed pro-inflammatory transcription factors while upregulating anti-inflammatory factors in splenocytes. CLE significantly reduced pro-inflammatory cytokine interferon-gamma (IFN‐γ) in serum samples, suggesting a neuroprotective effect by targeting pathways and overcoming BBB limitations. Conclusion: The observed immune modulation and neural protection in the CLE group suggest its potential for MS treatment. Further investigations are needed to elucidate mechanisms and explore clinical applications. The study highlights the promise of CLE-based therapy in addressing MS pathology.