Nickan Research Institute Immunopathologia Persa 2423-8015 11 2 2025 07 01 The effects of MCC950 on NLRP3 inflammasome and inflammatory biomarkers: a systematic review and meta-analysis on animal studies e43847 e43847 10.34172/ipp.2025.43847 EN Atousa Masoud https://orcid.org/0009-0007-7644-6876 Mojtaba Akbari https://orcid.org/0000-0002-5777-9612 Lida Jamal Ashini https://orcid.org/0009-0001-4891-2557 Roya Adelnia https://orcid.org/0000-0001-9888-207X Parisa Khodabandeh Shahraki https://orcid.org/0000-0002-4515-2636 Amirhossein Ramezani Ahmadi https://orcid.org/0000-0003-2581-963X Aghileh Panahi https://orcid.org/0000-0001-9679-3786 Journal Article 10.34172/ipp.2025.43847 2024 06 22 2024 12 27 Introduction: The NLRP3 inflammasome plays a key role in the regulation of inflammation and has been implicated in various inflammatory diseases. The present study evaluated the impact of MCC950 on NLRP3 and inflammatory biomarkers in systematic review and meta-analysis. Methods: Searches were performed across databases including Medline (PubMed), Scopus, Web of Science, and Embase. The pooled effects of MCC950 on NLRP3, TNF-alpha, interleukin (IL)-16, IL-18, and IL-1β were analyzed using a random effects model. Results: Twenty animal studies met the inclusion criteria. MCC950 did not significantly reduce NLRP3 (SMD=-0.88; 95% CI: -1.79,0.03; P=0.058) and TNF-alpha levels (SMD= -1.18; 95% CI: -2.45, 0.09; P=0.069). A significant reduction was observed in IL-18 (SMD= -1.22; 95% CI: -2.44, -0.00; P=0.049), IL-16 (SMD= -1.225; 95% CI: -2.779, 0.329; P=0.122) and IL-1β (SMD=-1.38; 95% CI: -2.44 to -0.32; P=0.011) concentrations after MCC950 treatment. Conclusion: MCC950 reduced serum levels of some inflammatory factors. However, NLRP3 did not change significantly. Further research is necessary to understand the function of pharmacological inhibition of NLRP3 activation. Registration: This study was conducted in accordance with the PRISMA checklist, and its protocol was registered on both the PROSPERO website (ID: CRD42024567477) and the Research Registry website (UIN: reviewregistry1887).