Nickan Research Institute Immunopathologia Persa 2423-8015 4 2 2018 07 01 The frequency of bla Verona imipenemase and bla imipenemase genes in clinical isolates of Pseudomonas aeruginosa in therapeutic centers of Zahedan e31 e31 10.15171/ipp.2018.31 EN Roghayeh Gholizadeh Doran Mahalleh Javad Dahmardeh Nahid Sepehri Rad Journal Article 10.15171/ipp.2018.31 2018 04 07 2018 08 03 Introduction: Pseudomonads are very important as an opportunistic bacterium among gram-negative bacteria. Class B beta-lactamases such as Verona imipenemase (VIM) and Imipenemase (IMP) are the most important contributors to the resistance of gram-negative bacteria like Pseudomonas aeruginosa. These enzymes were isolated from burn wounds in Zahedan due to extended spectrum beta-lactamase (ESBL) activity and high resistance to β-lactamase antibodies (IMP and VIM) in P. aeruginosa isolates. Objectives: This study was aimed to determine the frequency of VIM, IMP beta-lactamase genes in clinical isolates of P. aeruginosa isolated from therapeutic centers of Zahedan. Materials and Methods: In this descriptive cross-sectional study, 125 samples of P. aeruginosa from burn wounds were collected from patients who were hospitalized in Zahedan as well as were cultured. Suspected colonies of P. aeruginosa were identified using common biochemical methods. The Kirby Bayer method was used to measure antibiotic susceptibility, and then the minimum inhibitory concentration (MIC) of these strains was examined. The genotypes of the β-lactamase genes of IMP and VIM were investigated in polymerase chain reaction (PCR) strains. Results: In this study, 125 isolates of P. aeruginosa were found in 32 isolates (26%) producing ESBL. In general, the resistance of P. aeruginosa to the antibiotics of imipenem, piperacillin, ceftazidime, cefotaxime, ceftriaxone, levofloxacin, ciprofloxacin, gentamicin, amikacin, streptomycin, tetracycline, and carbenicillin were 88%, 93%, 89%, 98%, 97%, 89%, 84%, 82%, 97%, 91%, 90% and 95%, respectively. According to the PCR test, 32 isolates with ESBL enzymes were 43.75 and 18.75, respectively. Conclusion: The prevalence of ESBLs and antibiotic resistance in the burn injuries of hospital is high and requires such as antibiotic susceptibility test, rational administration of antibiotics and the control of beneficial factors. The results showed that most of the samples were resistant to the drug and the frequency of blaVIM genes was higher than blaIMP among the strains producing ESBLs.